Matrix attachment region elements have small and variable effects on transgene expression and stability in field-grown Populus.
Identifieur interne : 003869 ( Main/Exploration ); précédent : 003868; suivant : 003870Matrix attachment region elements have small and variable effects on transgene expression and stability in field-grown Populus.
Auteurs : Jingyi Li [États-Unis] ; Amy M. Brunner ; Richard Meilan ; Steven H. StraussSource :
- Plant biotechnology journal [ 1467-7644 ] ; 2008.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Amplification de gène (MeSH), Marqueurs génétiques (génétique), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Protéines à fluorescence verte (génétique), Régions d'ancrage à la matrice nucléaire (génétique), Régulation de l'expression des gènes végétaux (MeSH), Sondes d'ADN (MeSH), Tabac (génétique), Transformation génétique (MeSH), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (génétique).
- MESH :
- croissance et développement : Populus.
- génétique : Marqueurs génétiques, Populus, Protéines à fluorescence verte, Régions d'ancrage à la matrice nucléaire, Tabac, Végétaux génétiquement modifiés.
- métabolisme : Populus.
- Amorces ADN, Amplification de gène, Régulation de l'expression des gènes végétaux, Sondes d'ADN, Transformation génétique, Vecteurs génétiques.
English descriptors
- KwdEn :
- DNA Primers (MeSH), DNA Probes (MeSH), Gene Amplification (MeSH), Gene Expression Regulation, Plant (MeSH), Genetic Markers (genetics), Genetic Vectors (MeSH), Green Fluorescent Proteins (genetics), Matrix Attachment Regions (genetics), Plants, Genetically Modified (genetics), Populus (genetics), Populus (growth & development), Populus (metabolism), Tobacco (genetics), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Genetic Markers, Green Fluorescent Proteins.
- chemical : DNA Primers, DNA Probes.
- genetics : Matrix Attachment Regions, Plants, Genetically Modified, Populus, Tobacco.
- growth & development : Populus.
- metabolism : Populus.
- Gene Amplification, Gene Expression Regulation, Plant, Genetic Vectors, Transformation, Genetic.
Abstract
Matrix attachment regions (MARs) are thought to buffer transgenes from the influence of surrounding chromosomal sequences, and therefore to reduce transgene silencing and variation in expression. The statistical properties of more than 400 independent transgenic events produced in Populus, with and without flanking MAR elements from the tobacco root gene RB7, were analysed. The expression of two reporter genes in two poplar clones during three phases of vegetative growth, and the association of T-DNA characteristics with expression, was examined. It was found that MARs did not show a consistent effect on transgene expression levels; they had no effect on the green fluorescent protein (GFP) reporter gene, but reduced expression in the Basta resistance (BAR) reporter gene by 23%. The presence of MARs reduced expression variability within transformant populations, apparently by reducing the number of silenced or weakly expressing events. Transgene expression was highly stable over vegetative growth cycles that spanned 3 years of growth in the glasshouse and field, but MARs showed no association with the strength of correlations in expression over the years. Nonetheless, MARs increased the correlation in expression between a p35S::GFP and prbcS::BAR transgene linked on the same vector, but the effect was small and varied between the years. The presence of MARs had no effect on the transgene copy number, but was positively associated with T-DNA truncations, as well as with the formation of direct over inverted repeats at the same chromosomal locus.
DOI: 10.1111/j.1467-7652.2008.00369.x
PubMed: 19548343
Affiliations:
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Brunner</name></author><author><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:19548343</idno><idno type="pmid">19548343</idno><idno type="doi">10.1111/j.1467-7652.2008.00369.x</idno><idno type="wicri:Area/Main/Corpus">003529</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003529</idno><idno type="wicri:Area/Main/Curation">003529</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003529</idno><idno type="wicri:Area/Main/Exploration">003529</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Matrix attachment region elements have small and variable effects on transgene expression and stability in field-grown Populus.</title><author><name sortKey="Li, Jingyi" sort="Li, Jingyi" uniqKey="Li J" first="Jingyi" last="Li">Jingyi Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Brunner, Amy M" sort="Brunner, Amy M" uniqKey="Brunner A" first="Amy M" last="Brunner">Amy M. Brunner</name></author><author><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="ISSN">1467-7644</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA Primers (MeSH)</term><term>DNA Probes (MeSH)</term><term>Gene Amplification (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genetic Markers (genetics)</term><term>Genetic Vectors (MeSH)</term><term>Green Fluorescent Proteins (genetics)</term><term>Matrix Attachment Regions (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>Tobacco (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Amplification de gène (MeSH)</term><term>Marqueurs génétiques (génétique)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines à fluorescence verte (génétique)</term><term>Régions d'ancrage à la matrice nucléaire (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Sondes d'ADN (MeSH)</term><term>Tabac (génétique)</term><term>Transformation génétique (MeSH)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Genetic Markers</term><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>DNA Probes</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Matrix Attachment Regions</term><term>Plants, Genetically Modified</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Marqueurs génétiques</term><term>Populus</term><term>Protéines à fluorescence verte</term><term>Régions d'ancrage à la matrice nucléaire</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Amplification</term><term>Gene Expression Regulation, Plant</term><term>Genetic Vectors</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Amplification de gène</term><term>Régulation de l'expression des gènes végétaux</term><term>Sondes d'ADN</term><term>Transformation génétique</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Matrix attachment regions (MARs) are thought to buffer transgenes from the influence of surrounding chromosomal sequences, and therefore to reduce transgene silencing and variation in expression. The statistical properties of more than 400 independent transgenic events produced in Populus, with and without flanking MAR elements from the tobacco root gene RB7, were analysed. The expression of two reporter genes in two poplar clones during three phases of vegetative growth, and the association of T-DNA characteristics with expression, was examined. It was found that MARs did not show a consistent effect on transgene expression levels; they had no effect on the green fluorescent protein (GFP) reporter gene, but reduced expression in the Basta resistance (BAR) reporter gene by 23%. The presence of MARs reduced expression variability within transformant populations, apparently by reducing the number of silenced or weakly expressing events. Transgene expression was highly stable over vegetative growth cycles that spanned 3 years of growth in the glasshouse and field, but MARs showed no association with the strength of correlations in expression over the years. Nonetheless, MARs increased the correlation in expression between a p35S::GFP and prbcS::BAR transgene linked on the same vector, but the effect was small and varied between the years. The presence of MARs had no effect on the transgene copy number, but was positively associated with T-DNA truncations, as well as with the formation of direct over inverted repeats at the same chromosomal locus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19548343</PMID><DateCompleted><Year>2009</Year><Month>07</Month><Day>28</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>27</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1467-7644</ISSN><JournalIssue CitedMedium="Print"><Volume>6</Volume><Issue>9</Issue><PubDate><Year>2008</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Matrix attachment region elements have small and variable effects on transgene expression and stability in field-grown Populus.</ArticleTitle><Pagination><MedlinePgn>887-96</MedlinePgn></Pagination><Abstract><AbstractText>Matrix attachment regions (MARs) are thought to buffer transgenes from the influence of surrounding chromosomal sequences, and therefore to reduce transgene silencing and variation in expression. The statistical properties of more than 400 independent transgenic events produced in Populus, with and without flanking MAR elements from the tobacco root gene RB7, were analysed. The expression of two reporter genes in two poplar clones during three phases of vegetative growth, and the association of T-DNA characteristics with expression, was examined. It was found that MARs did not show a consistent effect on transgene expression levels; they had no effect on the green fluorescent protein (GFP) reporter gene, but reduced expression in the Basta resistance (BAR) reporter gene by 23%. The presence of MARs reduced expression variability within transformant populations, apparently by reducing the number of silenced or weakly expressing events. Transgene expression was highly stable over vegetative growth cycles that spanned 3 years of growth in the glasshouse and field, but MARs showed no association with the strength of correlations in expression over the years. Nonetheless, MARs increased the correlation in expression between a p35S::GFP and prbcS::BAR transgene linked on the same vector, but the effect was small and varied between the years. The presence of MARs had no effect on the transgene copy number, but was positively associated with T-DNA truncations, as well as with the formation of direct over inverted repeats at the same chromosomal locus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jingyi</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Oregon State University, Corvallis, OR 97331-5752, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brunner</LastName><ForeName>Amy M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Meilan</LastName><ForeName>Richard</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven H</ForeName><Initials>SH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015342">DNA Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015342" MajorTopicYN="N">DNA Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005784" MajorTopicYN="N">Gene Amplification</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045170" MajorTopicYN="N">Matrix Attachment Regions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>6</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>6</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>7</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19548343</ArticleId><ArticleId IdType="doi">10.1111/j.1467-7652.2008.00369.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Brunner, Amy M" sort="Brunner, Amy M" uniqKey="Brunner A" first="Amy M" last="Brunner">Amy M. Brunner</name><name sortKey="Meilan, Richard" sort="Meilan, Richard" uniqKey="Meilan R" first="Richard" last="Meilan">Richard Meilan</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Li, Jingyi" sort="Li, Jingyi" uniqKey="Li J" first="Jingyi" last="Li">Jingyi Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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